Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon

Abstract Microbiological safety of fish is a concern consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. one main agents foodborne diseases should be absent in animal products. Rapid accurate identification pathogens supply chain important for both quality assurance tracking infectious within chain. In this context, study aimed to evaluate equivalence two rapid detection tests, as alternative methods conventional method, well verify microbiological parameters commercially species Amazon biome. The plate count aerobic bacteria ranged from 7.76 x 104 8.71 107 CFU.g-1 mesophiles 1.70 106 4.27 108 psychrotrophic whereas maximum group microorganisms fresh CFU.g-1. Regarding Staphylococcus count, presented variations between 1.35 1.51 105 This represents unsatisfactory conditions handling, storage conservation species. immunoenzymatic molecular have been shown reliable, fast effective its high index agreement with method. We also emphasize convenience multiplex PCR application due sensitivity, specificity, speed accuracy detection.